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KMID : 0352720000240040196
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Journal of Ginseng Research
2000 Volume.24 No. 4 p.196 ~ p.201
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Protective Effects of Ginsenosides on Cyanide-induced Neurotoxicity in Cultured Rat Cerebellar Granule Cells
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Yeon Hee Seong/Yeon Hee Seong
Sang Bum Koh/Soon Ok Jo
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Abstract
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Effects of ginsenosides on NaCN-induced neuronal cell death were studied in cultured rat cerebellar granule cells. NaCN produced a concentration-dependent (1-10 ㎜) reduction of cell viability (measured by frypan blue exclusion test), that was blocked by N-methyl-D-aspartate receptor antagonist (MK-801) and L-type Ca2+ channel blocker (verapamil). Pretreatment with ginsenosides (Rb1, Rc, Re, Rf and Rg1) significantly decreased the neuronal cell death in a concentration range of 0.5∼5 ㎍/㎖. Ginsenosides Rb1 and Rc (5 ㎍/㎖) inhibited glutamate release into medium induced by NaCN (5 ㎜). NaCN (1 ㎜)-induced increase of [Ca2+]i, was significantly inhibited by the pretreatment of Rb1 and Rc (5 ㎍/㎖). Other ginsenosides caused relatively little inhibition on the elevation of glutamate release and of [Ca2+]i. These results suggest that the NaCN-induced neurotoxicity was related to a series of cell responses consisting of glutamate release and [Ca2+]i elevation via glutamate (NMDA and kainate) receptors and resultant cell death, and that ginsenosides, especially Rb1 and Rc, prevented the neuronal cell death by the blockade of the NaCN-induced Ca2+ influx.
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KEYWORD
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Ginsenosides, Rb1, Rc, Cerebellar granule neuron, NaCN, Ca2+ influx,
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